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Peptides. 2017 Feb;88:80-86. doi: 10.1016/j.peptides.2016.12.006. Epub 2016 Dec 21.

Synthetic peptide antigens derived from long-chain alpha-neurotoxins: Immunogenicity effect against elapid venoms.

Author information

1
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca Morelos 61500, Mexico.
2
Centro de Investigación en Dinámica Celular, IICBA, UAEM, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico.
3
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca Morelos 61500, Mexico. Electronic address: corzo@ibt.unam.mx.

Abstract

Three-finger toxins (3FTXs), especially α-neurotoxins, are the most poorly neutralized elapid snake toxins by current antivenoms. In this work, the conserved structural similarity and motif arrangements of long-chain α-neurotoxins led us to design peptides with consensus sequences. Eight long-chain α-neurotoxins (also known as Type II) were used to generate a consensus sequence from which two peptides were chemically synthesized, LCP1 and LCP2. Rabbit sera raised against them were able to generate partially-neutralizing antibodies, which delayed mice mortality in neutralization assays against Naja haje, Dendrospis polylepis and Ophiophagus hannah venoms.

KEYWORDS:

Elapid antivenom; Long-chain α-neurotoxins; Snakebites; Three-finger toxins

PMID:
28010961
DOI:
10.1016/j.peptides.2016.12.006
[Indexed for MEDLINE]

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