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Ultramicroscopy. 1989 Jul-Aug;30(3):281-97.

Magnification calibration and the determination of spherical virus diameters using cryo-microscopy.

Author information

1
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.

Abstract

The diameters of several frozen-hydrated, spherical viruses were determined using polyoma virus as either an external or an internal calibration standard. The methods described provide a reproducible and accurate way to calibrate microscope magnification. The measured diameters are in excellent agreement with respective measurements previously reported for aqueous samples at room temperature using X-ray diffraction methods. These results indicate that the native morphology and dimensions of biological macromolecules are better preserved in frozen-hydrated samples when compared with more conventional electron microscopy techniques such as negative-staining, metal shadowing or thin-sectioning.

PMID:
2800042
PMCID:
PMC4167718
[Indexed for MEDLINE]
Free PMC Article

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