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Cell Tissue Bank. 2017 Mar;18(1):17-25. doi: 10.1007/s10561-016-9605-2. Epub 2016 Dec 20.

Fresh and in vitro osteodifferentiated human amniotic membrane, alone or associated with an additional scaffold, does not induce ectopic bone formation in Balb/c mice.

Author information

1
Paediatric Surgery Service, University Hospital of Besancon, Besançon, France.
2
Novotec, Lyon, France.
3
Nanomedicine Lab, Imagery and Therapeutics (EA 4662), SFR FED 4234, University of Franche-Comté, Besançon, France.
4
Orthopaedic, Traumatology and Plastic Surgery Service, University Hospital of Besancon, Besançon, France.
5
Maxillofacial Surgery Service, University Hospital of Besancon, Besançon, France.
6
Inserm U957, Laboratory for Pathophysiology of Bone Resorption, Faculty of Medicine, University of Nantes, Nantes, France.
7
Department of Maxillofacial Surgery, Plastic - Reconstructive and Aesthetic Surgery, Hand Surgery, University Hospital of Dijon, Dijon, France.
8
Nanomedicine Lab, Imagery and Therapeutics (EA 4662), SFR FED 4234, University of Franche-Comté, Besançon, France. fgindraux@chu-besancon.fr.
9
Orthopaedic, Traumatology and Plastic Surgery Service, University Hospital of Besancon, Besançon, France. fgindraux@chu-besancon.fr.

Abstract

The human amniotic membrane (hAM) has been successfully used as a natural carrier containing amniotic mesenchymal stromal cells, epithelial cells and growth factors. It has a little or no immunogenicity, and possesses useful anti-microbial, anti-inflammatory, anti-fibrotic and analgesic properties. It has been used for many years in several indications for soft tissue repair. We previously reported that hAM represents a natural and preformed sheet containing highly potent stem cells, and could thus be used for bone repair. Indeed, native hAM possesses pre-osteoblastic potential that can easily be stimulated, even as far as mineralization, by means of in vitro osteogenic culture. However, cell culture induces damage to the tissue, as well as to cell phenotype and function. The aim of this study was to evaluate new bone formation by fresh and in vitro osteodifferentiated hAM, alone or associated with an additional scaffold presenting osteoinductive properties. Moreover, we also aimed to determine the effect of in vitro hAM pre-osteodifferentiation on its in vivo biocompatibility/tissue degradation. Results showed that neither fresh nor osteodifferentiated hAM induced ectopic bone formation, whether or not it was associated with the osteoinductive scaffold. Secondly, fresh and osteodifferentiated hAM presented similar in vivo tissue degradation, suggesting that in vitro hAM pre-osteodifferentiation did not influence its in vivo biocompatibility.

KEYWORDS:

Biocompatibility; Bone; Ectopic; Immune reaction; Mice; Osteogenic potential

PMID:
27999996
DOI:
10.1007/s10561-016-9605-2
[Indexed for MEDLINE]

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