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J Neurovirol. 2017 Jun;23(3):369-375. doi: 10.1007/s13365-016-0505-9. Epub 2016 Dec 19.

Cerebrospinal fluid biomarkers and HIV-associated neurocognitive disorders in HIV-infected individuals in Rakai, Uganda.

Author information

1
University of Minnesota, Minneapolis, MN.
2
Infectious Diseases Institute, Kampala, Uganda.
3
Rakai Health Sciences Program, Entebbe, Uganda.
4
Makerere University, Kampala, Uganda.
5
Johns Hopkins Bloomberg School of Public Health, Baltimore, MD.
6
University of North Carolina, Chapel Hill, NC.
7
Johns Hopkins University School of Medicine, Baltimore, MD.
#
Contributed equally

Abstract

In the USA, increased cerebrospinal fluid (CSF) inflammatory cytokines have been observed in antiretroviral therapy (ART)-naive, HIV-seropositive individuals with HIV-associated neurocognitive disorder (HAND). We characterized the relationship between HAND and CSF biomarker expression in ART-naive, HIV-seropositive individuals in Rakai, Uganda. We analyzed CSF of 78 HIV-seropositive, ART-naive Ugandan adults for 17 cytokines and 20 neurodegenerative biomarkers via Luminex multiplex assay. These adults underwent neurocognitive assessment to determine their degree of HAND. We compared biomarker concentrations between high and low CD4 groups and across HAND classifications, adjusting for multiple comparisons. Individuals with CD4 <200 cells/μL (N = 38) had elevated levels of CSF Interleukin (IL)-2, IL-12, granulocyte-macrophage colony-stimulating factor (GM-CSF), TNF-α, matrix metalloproteinase (MMP)-1, MMP-7, and S100 calcium-binding protein B (S100B) and lower levels of amyloid β42. Individuals with CD4 351-500 cells/μL (N = 40) had significantly higher CSF levels of interleukin (IL)-1β, amyloid β42, and soluble receptor for advanced glycation end products (sRAGE). Increasing levels of S100B, platelet-derived growth factor-AA (PDGF-AA), brain-derived neurotrophic factor (BDNF), and sRAGE were associated with decreased odds of mild neurocognitive disorder (n = 22) or HIV-associated dementia (n = 15) compared with normal function (n = 30) or asymptomatic neurocognitive impairment (n = 11). Increased levels of interferon (IFN)-γ were associated with increased odds of mild neurocognitive impairment or HIV-associated dementia relative to normal or asymptomatic neurocognitive impairment. Proinflammatory CSF cytokines, chemokines, and neurodegenerative biomarkers were present in increasing concentrations with advanced immunosuppression and may play a role in the development of HAND. The presence of select CNS biomarkers may also play a protective role in the development of HAND.

KEYWORDS:

Cytokine expression; Dementia; HIV-associated neurocognitive disorders; Inflammation; Neurodegenerative biomarkers

PMID:
27995575
PMCID:
PMC5441942
DOI:
10.1007/s13365-016-0505-9
[Indexed for MEDLINE]
Free PMC Article

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