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Nat Methods. 2017 Feb;14(2):145-148. doi: 10.1038/nmeth.4109. Epub 2016 Dec 19.

cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.

Author information

1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
2
Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
3
Department of Life Sciences, New York Institute of Technology, Old Westbury, New York, USA.

Abstract

The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.

PMID:
27992408
PMCID:
PMC5693259
DOI:
10.1038/nmeth.4109
[Indexed for MEDLINE]
Free PMC Article

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