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Elife. 2016 Dec 19;5. pii: e22866. doi: 10.7554/eLife.22866.

Semi-intact ex vivo approach to investigate spinal somatosensory circuits.

Author information

1
Department of Neurobiology, University of Pittsburgh, Pittsburgh, United States.
2
Pittsburgh Center for Pain Research, University of Pittsburgh, Pittsburgh, United States.

Abstract

The somatosensory input that gives rise to the perceptions of pain, itch, cold and heat are initially integrated in the superficial dorsal horn of the spinal cord. Here, we describe a new approach to investigate these neural circuits in mouse. This semi-intact somatosensory preparation enables recording from spinal output neurons, while precisely controlling somatosensory input, and simultaneously manipulating specific populations of spinal interneurons. Our findings suggest that spinal interneurons show distinct temporal and spatial tuning properties. We also show that modality selectivity - mechanical, heat and cold - can be assessed in both retrogradely labeled spinoparabrachial projection neurons and genetically labeled spinal interneurons. Finally, we demonstrate that interneuron connectivity can be determined via optogenetic activation of specific interneuron subtypes. This new approach may facilitate key conceptual advances in our understanding of the spinal somatosensory circuits in health and disease.

KEYWORDS:

dorsal horn; itch; mouse; neuroscience; optogenetics; pain; somatosensation; spinal cord

PMID:
27991851
PMCID:
PMC5214752
DOI:
10.7554/eLife.22866
[Indexed for MEDLINE]
Free PMC Article

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