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J Neurosci Res. 2017 Aug;95(8):1690-1699. doi: 10.1002/jnr.24000. Epub 2016 Dec 19.

Co-expression changes of lncRNAs and mRNAs in the cervical sympathetic ganglia in diabetic cardiac autonomic neuropathic rats.

Author information

1
Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
2
Undergraduate Student of Second Clinical Medical College, Medical College of Nanchang University, Nanchang, 330008, PR China.
3
Endocrine Department of Third Affiliated Hospital, Medical College of Nanchang University, Nanchang, 330008, PR China.
4
Department of Medical Genetics and Biology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
5
Department of Anatomy, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.

Abstract

Cardiac autonomic neuropathy in Type 2 diabetes (T2D) is often a devastating complication. Long non-coding RNAs (lncRNAs) have important effects on both normal development and disease pathogenesis. In this study, we explored the expression profiles of some lncRNAs involved in inflammation which may be co-expressed with messenger RNA (mRNA) in superior cervical and stellate ganglia after type 2 diabetic injuries. Total RNA isolated from 10 pairs of superior cervical and stellate ganglia in diabetic and normal male rats was hybridized to lncRNA arrays for detections. Pathway analysis indicated that the most significant gene ontology (GO) processes that were upregulated in diabetes were associated with immune response, cell migration, defense response, taxis, and chemotaxis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that most of the target genes of the lncRNAs were located in cytokine-cytokine receptor interactions, the chemokine signaling pathway and cell adhesion molecules, which were involved in T2D. Gene co-expression network construction showed that the co-expression network in the experimental rats consisted of 268 regulation edges among 105 lncRNAs and 11 mRNAs. Our studies demonstrated the co-expression profile of lncRNAs and mRNAs in diabetic cardiac autonomic ganglia, suggesting possible roles for multiple lncRNAs as potential targets for the development of therapeutic strategies or biomarkers for diabetic cardiac autonomic neuropathy.

KEYWORDS:

Long non-coding RNA; RRID: RGD_734476; RRID: SCR_004899; diabetic cardiac autonomic neuropathy; stellate ganglia; superior cervical ganglia

PMID:
27991686
DOI:
10.1002/jnr.24000
[Indexed for MEDLINE]

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