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J Extracell Vesicles. 2016 Dec 16;5:34299. doi: 10.3402/jev.v5.34299. eCollection 2016.

The International Society for Extracellular Vesicles launches the first massive open online course on extracellular vesicles.

Author information

1
Krefting Research Centre, Institution of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; cecilia.lasser@gu.se.
2
Institut Curie, PSL Research University, INSERM U932, Paris, France.
3
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.
4
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
5
Sue and Bill Gross Stem Cell Research Center, Chao Family Comprehensive Cancer Center, Edwards Lifesciences Center for Advanced Cardiovascular Technology, Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
6
Department of Biological Chemistry, University of California, Irvine, Irvine, CA, USA.
7
Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.
8
Department of Life Sciences, POSTECH, Pohang, South Korea.
9
Krefting Research Centre, Institution of Medicine at Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Abstract

The International Society for Extracellular Vesicles (ISEV) has organised its first educational online course for students and beginners in the field of extracellular vesicles (EVs). This course, "Basics of Extracellular Vesicles," uses recorded lectures from experts in the field and will be open for an unlimited number of participants. The course is divided into 5 modules and can be accessed at www.coursera.org/learn/extracellular-vesicles. The first module is an introduction to the field covering the nomenclature and history of EVs. Module 2 focuses on the biogenesis and uptake mechanisms of EVs, as well as their RNA, protein and lipid cargo. Module 3 covers the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Modules 4 and 5 present different isolation methods and characterisation techniques utilised in the EV field. Here, differential ultracentrifugation, size-exclusion chromatography, density gradient centrifugation, kit-based precipitation, electron microscopy, cryo-electron microscopy, flow cytometry, atomic-force microscopy and nanoparticle-tracking analysis are covered. This first massive open online course (MOOC) on EVs was launched on 15 August 2016 at the platform "Coursera" and is free of charge.

KEYWORDS:

Coursera; International Society for Extracellular Vesicles; education; exosomes; extracellular vesicles; massive open online course; microvesicles

PMID:
27989272
PMCID:
PMC5165052

Conflict of interest statement

and funding This course was funded by the International Society for Extracellular Vesicles and supported by grants for pedagogic development from the Sahlgrenska Academy, University of Gothenburg. YSG is the inventor of patents for using EVs as therapeutics, diagnostics and vaccines and is the founder of Aeon Medix and Rosetta Exosome and own stock in the company. JL is the co-owner of patents for using exosomes as therapeutics and is currently an employee of Codiak BioSciences, Inc. in parallel with his position at University of Gothenburg. Other authors declare no conflicts of interest.

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