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Immunity. 2017 Jan 17;46(1):148-161. doi: 10.1016/j.immuni.2016.11.005. Epub 2016 Dec 13.

Human Innate Lymphoid Cell Subsets Possess Tissue-Type Based Heterogeneity in Phenotype and Frequency.

Author information

1
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), 138648 Singapore. Electronic address: yannick_simoni@immunol.a-star.edu.sg.
2
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), 138648 Singapore.
3
Africa Health Research Institute (AHRI), 4001 Durban, South Africa; Department of Immunology and Microbiology, University of Copenhagen, 1165 Copenhagen, Denmark.
4
Division of Medical Oncology, National Cancer Centre Singapore (NCCS), 169610 Singapore.
5
Peter Medawar Building for Pathogen Research, University of Oxford, OX1 3SY Oxford, UK.
6
Department of Colorectal surgery, Singapore General Hospital, 169856 Singapore.
7
Department of Colorectal surgery, Singapore General Hospital, 169856 Singapore; Colorectal Practice, Mount Elizabeth Medical Centre, 228510 Singapore.
8
Department of Anatomical Pathology, Singapore General Hospital, 169608 Singapore.
9
Department of Hepatopancreatobiliary/Transplant Surgery, Singapore General Hospital, 169608 Singapore.
10
Department of Upper GI/Bariatric Surgery, Singapore General Hospital, 169608 Singapore.
11
KK Women's and Children's Hospital, Department of Reproductive Medicine, 229899 Singapore; Duke-NUS Graduate Medical School, 169857 Singapore.
12
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), 138648 Singapore; New York University School of Medicine, 10012 New york, USA.
13
Agency for Science, Technology and Research (A(∗)STAR), Institute of Molecular and Cell Biology (IMCB), 138673 Singapore.
14
Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 119228 Singapore.
15
Duke-NUS Graduate Medical School, 169857 Singapore; Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore, 119228 Singapore; Department of Medicine, National University Health System, 119228 Singapore; Perelman School of Medicine, University of Pennsylvania, 19104 USA.
16
KK Women's and Children's Hospital, Department of Otolaryngology, 229899 Singapore.
17
Africa Health Research Institute (AHRI), 4001 Durban, South Africa.
18
Division of Medical Oncology, National Cancer Centre Singapore (NCCS), 169610 Singapore; Agency for Science, Technology and Research (A(∗)STAR), Genome Institute of Singapore (GIS), 138672 Singapore.
19
Division of Medical Oncology, National Cancer Centre Singapore (NCCS), 169610 Singapore; Duke-NUS Graduate Medical School, 169857 Singapore; Agency for Science, Technology and Research (A(∗)STAR), Genome Institute of Singapore (GIS), 138672 Singapore.
20
Agency for Science, Technology and Research (A(∗)STAR), Singapore Immunology Network (SIgN), 138648 Singapore. Electronic address: evan_newell@immunol.a-star.edu.sg.

Abstract

Animal models have highlighted the importance of innate lymphoid cells (ILCs) in multiple immune responses. However, technical limitations have hampered adequate characterization of ILCs in humans. Here, we used mass cytometry including a broad range of surface markers and transcription factors to accurately identify and profile ILCs across healthy and inflamed tissue types. High dimensional analysis allowed for clear phenotypic delineation of ILC2 and ILC3 subsets. We were not able to detect ILC1 cells in any of the tissues assessed, however, we identified intra-epithelial (ie)ILC1-like cells that represent a broader category of NK cells in mucosal and non-mucosal pathological tissues. In addition, we have revealed the expression of phenotypic molecules that have not been previously described for ILCs. Our analysis shows that human ILCs are highly heterogeneous cell types between individuals and tissues. It also provides a global, comprehensive, and detailed description of ILC heterogeneity in humans across patients and tissues.

KEYWORDS:

CyTOF; ILC; ILC1; ILC2; ILC3; human; ieILC1

PMID:
27986455
DOI:
10.1016/j.immuni.2016.11.005
[Indexed for MEDLINE]
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