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Mol Cell. 2017 Jan 19;65(2):247-259. doi: 10.1016/j.molcel.2016.11.005. Epub 2016 Dec 13.

Mechanism of Ubiquitination and Deubiquitination in the Fanconi Anemia Pathway.

Author information

  • 1Genome Stability Unit, St. Vincent's Institute of Medical Research, Fitzroy, VIC 3065, Australia.
  • 2Genome Stability Unit, St. Vincent's Institute of Medical Research, Fitzroy, VIC 3065, Australia; Department of Medicine (St. Vincent's Health), The University of Melbourne, VIC 3010, Australia.
  • 3Architecture and Dynamics of Macromolecular Machines Laboratory, London Research Institute, South Mimms, Hertfordshire EN6 3LD, UK.
  • 4Molecular Genetics Unit, St. Vincent's Institute of Medical Research, Fitzroy, VIC 3065, Australia; Department of Medicine (St. Vincent's Health), The University of Melbourne, VIC 3010, Australia.
  • 5Genome Stability Unit, St. Vincent's Institute of Medical Research, Fitzroy, VIC 3065, Australia; Department of Medicine (St. Vincent's Health), The University of Melbourne, VIC 3010, Australia. Electronic address: adeans@svi.edu.au.

Abstract

Monoubiquitination and deubiquitination of FANCD2:FANCI heterodimer is central to DNA repair in a pathway that is defective in the cancer predisposition syndrome Fanconi anemia (FA). The "FA core complex" contains the RING-E3 ligase FANCL and seven other essential proteins that are mutated in various FA subtypes. Here, we purified recombinant FA core complex to reveal the function of these other proteins. The complex contains two spatially separate FANCL molecules that are dimerized by FANCB and FAAP100. FANCC and FANCE act as substrate receptors and restrict monoubiquitination to the FANCD2:FANCI heterodimer in only a DNA-bound form. FANCA and FANCG are dispensable for maximal in vitro ubiquitination. Finally, we show that the reversal of this reaction by the USP1:UAF1 deubiquitinase only occurs when DNA is disengaged. Our work reveals the mechanistic basis for temporal and spatial control of FANCD2:FANCI monoubiquitination that is critical for chemotherapy responses and prevention of Fanconi anemia.

KEYWORDS:

DNA repair; FANCB; FANCD2; Fanconi anemia; RING E3; core complex; deubiquitination; enzyme mechanism; monoubiquitination

PMID:
27986371
DOI:
10.1016/j.molcel.2016.11.005
[PubMed - in process]
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