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Sensors (Basel). 2016 Dec 14;16(12). pii: E2133.

Investigating Effects of Proteasome Inhibitor on Multiple Myeloma Cells Using Confocal Raman Microscopy.

Author information

1
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. jwkang76@mit.edu.
2
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. spsingh@mit.edu.
3
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. freddytn@mit.edu.
4
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. freddytn@mit.edu.
5
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. niyom@mit.edu.
6
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ysung4@uwm.edu.
7
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ptso@mit.edu.
8
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ptso@mit.edu.
9
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. ptso@mit.edu.
10
Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. rrdasari@mit.edu.

Abstract

Due to its label-free and non-destructive nature, applications of Raman spectroscopic imaging in monitoring therapeutic responses at the cellular level are growing. We have recently developed a high-speed confocal Raman microscopy system to image living biological specimens with high spatial resolution and sensitivity. In the present study, we have applied this system to monitor the effects of Bortezomib, a proteasome inhibitor drug, on multiple myeloma cells. Cluster imaging followed by spectral profiling suggest major differences in the nuclear and cytoplasmic contents of cells due to drug treatment that can be monitored with Raman spectroscopy. Spectra were also acquired from group of cells and feasibility of discrimination among treated and untreated cells using principal component analysis (PCA) was accessed. Findings support the feasibility of Raman technologies as an alternate, novel method for monitoring live cell dynamics with minimal external perturbation.

KEYWORDS:

Raman microscopy; Raman spectroscopy; cell imaging; therapeutic response monitoring

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