High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies

Anina Buchmann; Michael Eitel; Pierre Koch; Paul N Schwarz; Evi Stegmann; Wolfgang Wohlleben; Marcin Wolański; Michał Krawiec; Jolanta Zakrzewska-Czerwińska; Carmen Méndez; Alma Botas; Luz Elena Núñez; Francisco Morís; Jesus Cortés; Harald Gross

doi:10.1128/genomeA.01391-16

High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies

Genome Announc. 2016 Dec 15;4(6):e01391-16. doi: 10.1128/genomeA.01391-16.

Authors

Anina Buchmann^{1

2}, Michael Eitel³, Pierre Koch³, Paul N Schwarz⁴, Evi Stegmann^{2

4}, Wolfgang Wohlleben^{2

4}, Marcin Wolański⁵, Michał Krawiec⁵, Jolanta Zakrzewska-Czerwińska^{5

6}, Carmen Méndez⁷, Alma Botas⁷, Luz Elena Núñez⁸, Francisco Morís⁸, Jesus Cortés⁸, Harald Gross^{9

2}

Affiliations

¹ Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany.
² German Centre for Infection Research (DZIF), Partner Site Tübingen, Tübingen, Germany.
³ Department of Medicinal and Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany.
⁴ Department of Microbiology and Biotechnology, Interfaculty Institute of Microbiology and Infection Medicine, University of Tübingen, Tübingen, Germany.
⁵ Faculty of Biotechnology, University of Wrocław, Wrocław, Poland.
⁶ Ludwik Hirszfeld Insitute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland.
⁷ Departamento de Biología Funcional e Instituto Universitario de Oncología del Principado de Asturias, Universidad de Oviedo, Oviedo, Spain.
⁸ EntreChem S.L., Oviedo, Spain.
⁹ Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany harald.gross@uni-tuebingen.de.

Abstract

The bacterium Nocardia terpenica IFM 0406 is known as the producer of the immunosuppressant brasilicardin A. Here, we report the completely sequenced genome of strain IFM 0406, which facilitates the heterologous expression of the brasilicardin biosynthetic gene cluster but also unveils the intriguing biosynthetic capacity of the strain to produce secondary metabolites.