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Proteomics. 2017 Mar;17(5). doi: 10.1002/pmic.201600397. Epub 2017 Feb 1.

N-glycoprotein surfaceome of human induced pluripotent stem cell derived hepatic endoderm.

Author information

1
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA.
2
Present address: National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD, USA.
3
Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.

Abstract

Using cell surface capture technology, the cell surface N-glycoproteome of human-induced pluripotent stem cell derived hepatic endoderm cells was assessed. Altogether, 395 cell surface N-glycoproteins were identified, represented by 1273 N-glycopeptides. This study identified N-glycoproteins that are not predicted to be localized to the cell surface and provides experimental data that assist in resolving ambiguous or incorrectly annotated transmembrane topology annotations. In a proof-of-concept analysis, combining these data with other cell surface proteome datasets is useful for identifying potentially cell type and lineage restricted markers and drug targets to advance the use of stem cell technologies for mechanistic developmental studies, disease modeling, drug discovery, and regenerative medicine.

KEYWORDS:

Cell surface proteins; Glycoproteomics; Hepatic endoderm; N-glycoproteins; Plasma membrane

PMID:
27966262
PMCID:
PMC5340625
DOI:
10.1002/pmic.201600397
[Indexed for MEDLINE]
Free PMC Article

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