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Folia Histochem Cytobiol. 2016;54(4):186-192. doi: 10.5603/FHC.a2016.0024. Epub 2016 Dec 14.

Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody.

Author information

1
Gemacbio, Saint Jean d'Illac, France Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), Talence, France Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems, University of Salamanca, Salamanca, Spain. mangasam@usal.es.

Abstract

INTRODUCTION:

The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.

MATERIAL AND METHODS:

According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10-8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.

RESULTS:

Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2-3 long (50-100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.

CONCLUSIONS:

The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.

KEYWORDS:

IHC; brain; lateral septal nucleus; new antibody; pantothenic acid; rat; vitamin B5

PMID:
27966211
DOI:
10.5603/FHC.a2016.0024
[Indexed for MEDLINE]
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