Format

Send to

Choose Destination
Genome Res. 2017 Feb;27(2):289-299. doi: 10.1101/gr.215293.116. Epub 2016 Dec 13.

Large-scale reduction of the Bacillus subtilis genome: consequences for the transcriptional network, resource allocation, and metabolism.

Author information

1
Department of General Microbiology, Georg-August-University Göttingen, 37077 Göttingen, Germany.
2
Institute for Industrial Genetics, University of Stuttgart, 70569 Stuttgart, Germany.
3
Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, 17487 Greifswald, Germany.
4
Department of Plant Biochemistry, Georg-August-University Göttingen, 37077 Göttingen, Germany.
5
Department of Genomic and Applied Microbiology, Göttingen Genomics Laboratory, Georg-August-University Göttingen, 37077 Göttingen, Germany.
6
MaIAGE, INRA Université Paris-Saclay, 78350 Jouy-en-Josas, France.
7
Georg-August-University, Göttingen Center for Molecular Biosciences (GZMB), 37077 Göttingen, Germany.
8
Institute for Nonlinear Dynamics, Georg-August-University Göttingen, 37077 Göttingen, Germany.

Abstract

Understanding cellular life requires a comprehensive knowledge of the essential cellular functions, the components involved, and their interactions. Minimized genomes are an important tool to gain this knowledge. We have constructed strains of the model bacterium, Bacillus subtilis, whose genomes have been reduced by ∼36%. These strains are fully viable, and their growth rates in complex medium are comparable to those of wild type strains. An in-depth multi-omics analysis of the genome reduced strains revealed how the deletions affect the transcription regulatory network of the cell, translation resource allocation, and metabolism. A comparison of gene counts and resource allocation demonstrates drastic differences in the two parameters, with 50% of the genes using as little as 10% of translation capacity, whereas the 6% essential genes require 57% of the translation resources. Taken together, the results are a valuable resource on gene dispensability in B. subtilis, and they suggest the roads to further genome reduction to approach the final aim of a minimal cell in which all functions are understood.

PMID:
27965289
PMCID:
PMC5287234
DOI:
10.1101/gr.215293.116
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center