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Arch Physiol Biochem. 2017 May;123(2):78-92. doi: 10.1080/13813455.2016.1251945. Epub 2016 Dec 14.

Date fruits inhibit hepatocyte apoptosis and modulate the expression of hepatocyte growth factor, cytochrome P450 2E1 and heme oxygenase-1 in carbon tetrachloride-induced liver fibrosis.

Author information

1
a Department of Pharmacology and Toxicology , College of Pharmacy, King Saud University , Riyadh , Kingdom of Saudi Arabia.
2
b Department of Biochemistry , College of Pharmacy, Mansoura University , Mansoura , Egypt , and.
3
c Anatomy Department , Faculty of Medicine, King Saud University , Riyadh , Kingdom of Saudi Arabia.

Abstract

CONTEXT:

Date fruits have protective effects against liver fibrosis; however their anti-apoptotic effects have not been investigated.

OBJECTIVE:

To investigate the modulating effects of date fruits on pro- and anti-apoptotic markers, cytochrome P450 2E1 (CYP2E1) and hepatocyte growth factor (HGF) in liver fibrosis.

MATERIALS AND METHODS:

Liver fibrosis was induced by injection of carbon tetrachloride (CCl4) for eight weeks. Date flesh extract (DFE) and pits extract (DPE) were taken daily concomitant with CCl4. Hepatocyte apoptosis was determined by measuring the expression of Fas, caspase-3, Bax, Bcl2 and hemeoxygenase-1 (HO-1). Hepatic levels of HGF and CYP2E1 were determined.

RESULTS:

Treatment with DFE and DPE significantly attenuated the elevated levels of Fas, caspase 3, Bax and CYP2E1 induced by CCl4. In addition, they alleviated the reduction in Bcl2, HGF and HO-1, the cytoprotective and anti-apoptotic factors in liver. Conclusions DFE and DPE treatment can ameliorate liver fibrosis by inhibiting hepatocyte apoptosis.

KEYWORDS:

Liver fibrosis; apoptosis; casapase-3; cytochrome P450 2E1; hepatocyte growth factor

PMID:
27960551
DOI:
10.1080/13813455.2016.1251945
[Indexed for MEDLINE]

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