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Mol Med Rep. 2017 Jan;15(1):111-116. doi: 10.3892/mmr.2016.6026. Epub 2016 Dec 12.

Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging.

Author information

1
Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
2
Department of Burn and Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
3
Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
4
The Central Laboratory, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
5
Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.

Abstract

Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL‑PS‑associated inhibition of ultraviolet B (UVB)‑induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence‑associated β-galactosidase staining (SA‑β‑gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) ‑1 and C‑telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP‑1 protein expression, and increased cellular ROS levels compared with non‑exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL‑PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP‑1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL‑PS untreated cells. These results demonstrate that GL‑PS protects fibroblasts against photoaging by eliminating UVB‑induced ROS. This finding indicates GL‑PS treatment may serve as a novel strategy for antiphotoaging.

PMID:
27959406
PMCID:
PMC5355704
DOI:
10.3892/mmr.2016.6026
[Indexed for MEDLINE]
Free PMC Article

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