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Sci Rep. 2016 Dec 13;6:38828. doi: 10.1038/srep38828.

General calibration of microbial growth in microplate readers.

Author information

1
Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, Roger Land Building, Alexander Crum Brown Road, Edinburgh, EH9 3FF, United Kingdom.
2
Centre for Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, CH Waddington Building, Mayfield Road, Edinburgh EH9 3JD, UK.

Abstract

Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from studies of antibiotic efficacy to investigations of growth under different nutritional or stress environments, to characterization of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions, and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.

PMID:
27958314
PMCID:
PMC5153849
DOI:
10.1038/srep38828
[Indexed for MEDLINE]
Free PMC Article

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