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JCI Insight. 2016 Dec 8;1(20):e88242.

Tissue distribution and clonal diversity of the T and B cell repertoire in type 1 diabetes.

Author information

1
Department of Pathology, Immunology and Laboratory Medicine, University of Florida Diabetes Institute, Gainesville, Florida, USA.
2
Adaptive Biotechnologies Corporation, Seattle, Washington, USA.
3
Department of Information Sciences, City of Hope National Medical Center, Duarte, California, USA.
4
Barbara Davis Center for Childhood Diabetes and.
5
Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, Colorado, USA.
6
Diabetes Research Institute and Departments of Medicine, Microbiology, and Immunology, University of Miami Miller School of Medicine, Miami, Florida, USA.
7
Adaptive Biotechnologies Corporation, Seattle, Washington, USA.; Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.

Abstract

The adaptive immune repertoire plays a critical role in type 1 diabetes (T1D) pathogenesis. However, efforts to characterize B cell and T cell receptor (TCR) profiles in T1D subjects have been largely limited to peripheral blood sampling and restricted to known antigens. To address this, we collected pancreatic draining lymph nodes (pLN), "irrelevant" nonpancreatic draining lymph nodes, peripheral blood mononuclear cells (PBMC), and splenocytes from T1D subjects (n = 18) and control donors (n = 9) as well as pancreatic islets from 1 T1D patient; from these tissues, we collected purified CD4+ conventional T cells (Tconv), CD4+ Treg, CD8+ T cells, and B cells. By conducting high-throughput immunosequencing of the TCR β chain (TRB) and B cell receptor (BCR) immunoglobulin heavy chain (IGH) on these samples, we sought to analyze the molecular signature of the lymphocyte populations within these tissues and of T1D. Ultimately, we observed a highly tissue-restricted CD4+ repertoire, while up to 24% of CD8+ clones were shared among tissues. We surveyed our data set for previously described proinsulin- and glutamic acid decarboxylase 65-reactive (GAD65-reactive) receptors, and interestingly, we observed a TRB with homology to a known GAD65-reactive TCR (clone GAD4.13) present in 7 T1D donors (38.9%), representing >25% of all productive TRB within Tconv isolated from the pLN of 1 T1D subject. These data demonstrate diverse receptor signatures at the nucleotide level and enriched autoreactive clones at the amino acid level, supporting the utility of coupling immunosequencing data with knowledge of characterized autoreactive receptors.

PMID:
27942583
PMCID:
PMC5135280
DOI:
10.1172/jci.insight.88242
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

M. Vignali, R.O. Emerson, and E. Yusko are employees of and have equity ownership in Adaptive Biotechnologies. H.S. Robins is an employee, has equity ownership, patents, and royalties with Adaptive Biotechnologies.

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