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BMC Genomics. 2016 Dec 9;17(1):1015.

Globally distributed root endophyte Phialocephala subalpina links pathogenic and saprophytic lifestyles.

Author information

1
Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, ETH Zürich, 8092, Zürich, Switzerland.
2
Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany.
3
Department of Genome-oriented Bioinformatics, Technische Universität München, Wissenschaftszentrum Weihenstephan, 85354, Freising, Germany.
4
Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Berne, Baltzerstrasse 6, 3012, Bern, Switzerland.
5
Architecture et Fonction des Macromolécules Biologiques (AFMB), UMR 7257 CNRS, Université Aix-Marseille, 163 Avenue de Luminy, 13288, Marseille, France.
6
DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA, 94598, USA.
7
Friedrich-Schiller-Universität, Pharmazeutische Mikrobiologie, Winzerlaer Strasse 2, 07745, Jena, Germany.
8
Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, ETH Zürich, 8092, Zürich, Switzerland. christoph.gruenig@microsynth.ch.
9
Microsynth AG, Schützenstrasse 15, 9436, Balgach, Switzerland. christoph.gruenig@microsynth.ch.

Abstract

BACKGROUND:

Whereas an increasing number of pathogenic and mutualistic ascomycetous species were sequenced in the past decade, species showing a seemingly neutral association such as root endophytes received less attention. In the present study, the genome of Phialocephala subalpina, the most frequent species of the Phialocephala fortinii s.l. - Acephala applanata species complex, was sequenced for insight in the genome structure and gene inventory of these wide-spread root endophytes.

RESULTS:

The genome of P. subalpina was sequenced using Roche/454 GS FLX technology and a whole genome shotgun strategy. The assembly resulted in 205 scaffolds and a genome size of 69.7 Mb. The expanded genome size in P. subalpina was not due to the proliferation of transposable elements or other repeats, as is the case with other ascomycetous genomes. Instead, P. subalpina revealed an expanded gene inventory that includes 20,173 gene models. Comparative genome analysis of P. subalpina with 13 ascomycetes shows that P. subalpina uses a versatile gene inventory including genes specific for pathogens and saprophytes. Moreover, the gene inventory for carbohydrate active enzymes (CAZymes) was expanded including genes involved in degradation of biopolymers, such as pectin, hemicellulose, cellulose and lignin.

CONCLUSIONS:

The analysis of a globally distributed root endophyte allowed detailed insights in the gene inventory and genome organization of a yet largely neglected group of organisms. We showed that the ubiquitous root endophyte P. subalpina has a broad gene inventory that links pathogenic and saprophytic lifestyles.

KEYWORDS:

Comparative genomics; Lifestyle; Parasitism-mutualism continuum; Root endophyte; Species complex

PMID:
27938347
PMCID:
PMC5148876
DOI:
10.1186/s12864-016-3369-8
[Indexed for MEDLINE]
Free PMC Article

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