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Transcription. 2017 Jan;8(1):55-60. doi: 10.1080/21541264.2016.1265701. Epub 2016 Dec 9.

Structural dynamics and DNA interaction of human TFIID.

Author information

1
a Molecular and Cell Biology Department and QB3 Institute , UC Berkeley , CA , USA.
2
b Howard Hughes Medical Institute , UC Berkeley , CA , USA.
3
c Molecular Biophysics and Integrative Bio-Imaging Division , Lawrence Berkeley National Lab , CA , USA.
4
d Biophysics Graduate Group , UC Berkeley , CA , USA.

Abstract

TFIID is a large protein complex required for the recognition and binding of eukaryotic gene core promoter sequences and for the recruitment of the rest of the general transcription factors involved in initiation of eukaryotic protein gene transcription. Cryo-electron microscopy studies have demonstrated the conformational complexity of human TFIID, where one-third of the mass of the complex can shift its position by well over 100 Å. This conformational plasticity appears to be linked to the capacity of TFIID to bind DNA, and suggests how it would allow both the recognition of different core promoter elements and the tuning of its binding affinity by regulatory factors.

KEYWORDS:

conformational heterogeneity; cryo-EM; pre-initiation complex; transcription initiation

PMID:
27935424
PMCID:
PMC5279711
DOI:
10.1080/21541264.2016.1265701
[Indexed for MEDLINE]
Free PMC Article

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