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Cell Res. 2017 Feb;27(2):226-240. doi: 10.1038/cr.2016.147. Epub 2016 Dec 9.

A pair of transposon-derived proteins function in a histone acetyltransferase complex for active DNA demethylation.

Author information

1
Shanghai Center for Plant Stress Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, China.
2
Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette, IN 47907, USA.
3
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
4
The State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Agricultural Research Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, China.
5
Biotechnology Section, Indian Institute of Rice Research (IIRR), Hyderabad, India.
6
College of Agriculture, Henan University of Science and Technology, Luoyang, Henan 471026, China.

Abstract

Transposons are generally kept silent by epigenetic mechanisms including DNA methylation. Here, we identified a pair of Harbinger transposon-derived proteins (HDPs), HDP1 and HDP2, as anti-silencing factors in Arabidopsis. hdp1 and hdp2 mutants displayed an enhanced silencing of transgenes and some transposons. Phylogenetic analyses revealed that HDP1 and HDP2 were co-domesticated from the Harbinger transposon-encoded transposase and DNA-binding protein, respectively. HDP1 interacts with HDP2 in the nucleus, analogous to their transposon counterparts. Moreover, HDP1 and HDP2 are associated with IDM1, IDM2, IDM3 and MBD7 that constitute a histone acetyltransferase complex functioning in DNA demethylation. HDP2 and the methyl-DNA-binding protein MBD7 share a large set of common genomic binding sites, indicating that they jointly determine the target specificity of the histone acetyltransferase complex. Thus, our data revealed that HDP1 and HDP2 constitute a functional module that has been recruited to a histone acetyltransferase complex to prevent DNA hypermethylation and epigenetic silencing.

PMID:
27934869
PMCID:
PMC5339849
DOI:
10.1038/cr.2016.147
[Indexed for MEDLINE]
Free PMC Article

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