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Org Lett. 2016 Nov 18;18(22):5796-5799. Epub 2016 Nov 4.

Preparation of Protein Conjugates via Homobifunctional Diselenoester Cross-Linker.

Author information

1
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, CCNU-uOttawa Joint Research Centre, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China.

Abstract

Adipic acid diselenoester was developed as an efficient cross-linker for covalent protein conjugation with a variety of small molecular haptens, including mono- and disaccharides, peptide, fluorescence dye, and nicotine. Compared to the counterparts of N-hydroxysuccinimide (NHS) and p-nitrophenyl (PNP) linkers, the diselenoester linker demonstrates improved balance between reactivity and stability and coupling of haptens to proteins under mild conditions with high incorporation efficiency.

PMID:
27934486
DOI:
10.1021/acs.orglett.6b02568
[Indexed for MEDLINE]

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