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Regen Med. 2017 Mar;12(2):125-141. doi: 10.2217/rme-2016-0142. Epub 2016 Dec 7.

Hydrogen sulfide enhances pancreatic β-cell differentiation from human tooth under normal and glucotoxic conditions.

Author information

1
Department of Oral Health, Nippon Dental University, School of Life Dentistry at Tokyo, 1-9-20 Chiyoda-ku, 102-8159 Tokyo, Japan.

Abstract

AIM:

Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of H2S on glucotoxicity.

MATERIALS & METHODS:

DPSCs were differentiated with media containing 5.5 or 25.0 mM glucose, exposed to 1 ng/ml H2S. Glucotoxicity, expression of β-cell markers, INS, PDX1 and GLUT2, and PI3K/AKT pathway were assessed.

RESULTS:

H2S exposure increased insulin and C-peptide, and protected DPSC-derived pancreatic β-like cells from glucotoxicity and upregulated INS, PDX1 and GLUT2, and genes of PI3K/AKT pathway.

CONCLUSION:

H2S improved effects of glucotoxicity on β-like cells via PI3K/AKT pathway. The protocol for pancreatic β-cell differentiation might have applications in regenerative medicine rather than swine pancreas transplantation.

KEYWORDS:

glucotoxicity; hydrogen sulfide; pancreatic β-like cells

PMID:
27925870
DOI:
10.2217/rme-2016-0142
[Indexed for MEDLINE]

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