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Adv Healthc Mater. 2016 Dec;5(23):2972-2982. doi: 10.1002/adhm.201600857. Epub 2016 Nov 7.

Quantification of Type, Timing, and Extent of Cell Body and Nucleus Deformations Caused by the Dimensions and Hydrophilicity of Square Prism Micropillars.

Author information

1
Graduate Department of Biotechnology, Middle East Technical University (METU), Ankara, 06800, Turkey.
2
BIOMATEN, Center of Excellence in Biomaterials and Tissue Engineering, Middle East Technical University (METU), Ankara, 06800, Turkey.
3
Institute of Applied Mathematics, Middle East Technical University (METU), Ankara, 06800, Turkey.
4
Bio-Acoustic-MEMs in Medicine (BAMM) Laboratory, Stanford School of Medicine, Palo Alto, CA, 94394, USA.
5
Department of Chemistry, Middle East Technical University (METU), Ankara, 06800, Turkey.
6
Department of Biological Sciences, Middle East Technical University (METU), Ankara, 06800, Turkey.

Abstract

Novel digital analysis strategies are developed for the quantification of changes in the cytoskeletal and nuclear morphologies of mesenchymal stem cells cultured on micropillars. Severe deformations of nucleus and distinct conformational changes of cell body ranging from extensive elongation to branching are visualized and quantified. These deformations are caused mainly by the dimensions and hydrophilicity of the micropillars.

KEYWORDS:

conformational changes; cytoskeleton; micropillars; nucleus deformations; oxygen plasma modification

PMID:
27925459
DOI:
10.1002/adhm.201600857
[Indexed for MEDLINE]

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