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Nat Commun. 2016 Dec 7;7:13600. doi: 10.1038/ncomms13600.

Mature IgM-expressing plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge.

Author information

1
CIRI, INSERM, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, École Normale Supérieure de Lyon, Univ. Lyon, 21 Avenue Tony Garnier, 69007 Lyon, France.
2
AltraBio SAS, 30 rue Pre Gaudry, 69007 Lyon, France.
3
INSERM SFR Biosciences Gerland, UMS3444/US8, 50 Avenue Tony Garnier, 69007 Lyon, France.
4
eBioscience, An Affymetrix Company, 140 bis Rue de Rennes, 75006 Paris, France.
5
Institute of Immunology, University Hospital, Albert Einstein Allee 11, Ulm 89073, Germany.
6
INSERM, UMR917, F-35043 Rennes, France.
7
Pôle de Biologie, Centre Hospitalier Universitaire, 35033 Rennes, France.
8
Université de Rennes 1, F-35065 Rennes, France.
9
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia.
10
Department of Medical Biology, University of Melbourne, Parkville, Victoria 3010, Australia.

Abstract

Dogma holds that plasma cells, as opposed to B cells, cannot bind antigen because they have switched from expression of membrane-bound immunoglobulins (Ig) that constitute the B-cell receptor (BCR) to production of the secreted form of immunoglobulins. Here we compare the phenotypical and functional attributes of plasma cells generated by the T-cell-dependent and T-cell-independent forms of the hapten NP. We show that the nature of the secreted Ig isotype, rather than the chemical structure of the immunizing antigen, defines two functionally distinct populations of plasma cells. Fully mature IgM-expressing plasma cells resident in the bone marrow retain expression of a functional BCR, whereas their IgG+ counterparts do not. Antigen boost modifies the gene expression profile of IgM+ plasma cells and initiates a cytokine production program, characterized by upregulation of CCL5 and IL-10. Our results demonstrate that IgM-expressing plasma cells can sense antigen and acquire competence for cytokine production upon antigenic challenge.

PMID:
27924814
PMCID:
PMC5150646
DOI:
10.1038/ncomms13600
[Indexed for MEDLINE]
Free PMC Article

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