Format

Send to

Choose Destination
J Am Soc Mass Spectrom. 2017 Feb;28(2):389-392. doi: 10.1007/s13361-016-1552-4. Epub 2016 Dec 6.

Incorporation of a Reporter Peptide in FPOP Compensates for Adventitious Scavengers and Permits Time-Dependent Measurements.

Author information

1
Department of Chemistry, Washington University in St. Louis, St. Louis, MO, 63130, USA.
2
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, 01605, USA.
3
Department of Chemistry, Washington University in St. Louis, St. Louis, MO, 63130, USA. mgross@wustl.edu.

Abstract

Incorporation of a reporter peptide in solutions submitted to fast photochemical oxidation of proteins (FPOP) allows for the correction of adventitious scavengers and enables the normalization and comparison of time-dependent results. Reporters will also be useful in differential experiments to control for the inclusion of a radical-reactive species. This incorporation provides a simple and quick check of radical dosage and allows comparison of FPOP results from day-to-day and lab-to-lab. Use of a reporter peptide in the FPOP workflow requires no additional measurements or spectrometers while building a more quantitative FPOP platform. It requires only measurement of the extent of reporter-peptide modification in a LC/MS/MS run, which is performed by using either data-dependent scanning or an inclusion list. Graphical Abstract ᅟ.

KEYWORDS:

Adventitious radical scavenger; FPOP; Mass spectrometry; Reporter; SOD1

PMID:
27924496
PMCID:
PMC5233597
DOI:
10.1007/s13361-016-1552-4
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center