Format

Send to

Choose Destination
Dev Cell. 2016 Dec 5;39(5):585-596. doi: 10.1016/j.devcel.2016.09.031. Epub 2016 Oct 27.

Establishment of Expression in the SHORTROOT-SCARECROW Transcriptional Cascade through Opposing Activities of Both Activators and Repressors.

Author information

1
Department of Biology, Duke University, Durham, NC 27708, USA.
2
Department of Plant Biology and Genome Center, University of California Davis, Davis, CA 95616, USA.
3
Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061, USA.
4
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA.
5
Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA; Center for Genomic and Computational Biology, Duke University, Durham, NC 27708, USA.
6
Department of Biology, Duke University, Durham, NC 27708, USA; Center for Genomic and Computational Biology, Duke University, Durham, NC 27708, USA; Department of Computer Science, Duke University, Durham, NC 27708, USA.
7
Center for Genomic and Computational Biology, Duke University, Durham, NC 27708, USA; Department of Computer Science, Duke University, Durham, NC 27708, USA; Department of Biostatistics and Bioinformatics, Duke University, Durham, NC 27710, USA.
8
Department of Biology, Duke University, Durham, NC 27708, USA; Howard Hughes Medical Institute, Duke University, Durham, NC 27708, USA. Electronic address: philip.benfey@duke.edu.

Abstract

Tissue-specific gene expression is often thought to arise from spatially restricted transcriptional cascades. However, it is unclear how expression is established at the top of these cascades in the absence of pre-existing specificity. We generated a transcriptional network to explore how transcription factor expression is established in the Arabidopsis thaliana root ground tissue. Regulators of the SHORTROOT-SCARECROW transcriptional cascade were validated in planta. At the top of this cascade, we identified both activators and repressors of SHORTROOT. The aggregate spatial expression of these regulators is not sufficient to predict transcriptional specificity. Instead, modeling, transcriptional reporters, and synthetic promoters support a mechanism whereby expression at the top of the SHORTROOT-SCARECROW cascade is established through opposing activities of activators and repressors.

KEYWORDS:

Arabidopsis; SCARECROW; SHORTROOT; roots; transcription factor

PMID:
27923776
PMCID:
PMC5349323
DOI:
10.1016/j.devcel.2016.09.031
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center