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Diagn Microbiol Infect Dis. 2017 Mar;87(3):203-206. doi: 10.1016/j.diagmicrobio.2016.11.013. Epub 2016 Nov 25.

Development of a multiplex taqMan real-time PCR assay for typing of Mycoplasma pneumoniae based on type-specific indels identified through whole genome sequencing.

Author information

1
Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
2
Division of Bacterial Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. Electronic address: jwinchell@cdc.gov.

Abstract

We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.

KEYWORDS:

M. pneumoniae; Next generation sequencing; P1 typing; Real-time PCR

[Indexed for MEDLINE]

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