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Bioanalysis. 2017 Jan;9(1):9-19.

Lipoprotein profiling methodology based on determination of apolipoprotein concentration.

Author information

1
Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812 8582, Japan.
2
Department of Bioinformatic Engineering, Graduate School of Information Science & Technology, Osaka University, 1-5 Yamadaoka, Suita, Osaka 565 0871, Japan.
3
Institute for Experimental Animals, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650 0017, Japan.
4
Division of Comparative Pathophysiology, Department of Physiology & Cell Biology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650 0017, Japan.
5
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565 0871, Japan.

Abstract

AIM:

Abnormal lipid metabolism results in the alteration of lipid compositions in lipoproteins; therefore an accurate and quantitative analytical approach is required for the detailed structural characterization of lipoproteins. However, the specific lipid composition of each lipoprotein particle is poorly understood.

MATERIALS & METHODS:

Lipid composition of very-low-density lipoprotein and low-density lipoprotein particles derived from myocardial infarction-prone rabbits was determined by normalization of lipidomics data using apoB-100 levels.

RESULTS:

The ratio of lipid levels between very-low-density lipoprotein and low-density lipoprotein particles was different according to not only lipid classes, but also phosphatidylethanolamine subclasses by applying our developed methodology to myocardial infarction-prone rabbits.

CONCLUSION:

Our novel analytical approach represents to be a potentially useful tool to obtain particle-specific lipid components of lipoproteins.

KEYWORDS:

apolipoprotein; lipoprotein; targeted proteomics

PMID:
27921455
DOI:
10.4155/bio-2016-0234
[Indexed for MEDLINE]

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