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Monoclon Antib Immunodiagn Immunother. 2016 Dec;35(6):304-306. doi: 10.1089/mab.2016.0040. Epub 2016 Dec 5.

Podoplanin Expression in Canine Melanoma.

Author information

1
1 Department of Regional Innovation, Tohoku University Graduate School of Medicine , Miyagi, Japan .
2
2 North Lab , Hokkaido, Japan .
3
3 Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University , Hokkaido, Japan .

Abstract

A type I transmembrane protein, podoplanin (PDPN), is expressed in several normal cells such as lymphatic endothelial cells or pulmonary type I alveolar cells. We recently demonstrated that anticanine PDPN monoclonal antibody (mAb), PMab-38, recognizes canine PDPN of squamous cell carcinomas, but does not react with lymphatic endothelial cells. Herein, we investigated whether PMab-38 reacts with canine melanoma. PMab-38 reacted with 90% of melanoma cells (9/10 cases) using immunohistochemistry. Of interest, PMab-38 stained the lymphatic endothelial cells and cancer-associated fibroblasts in melanoma tissues, although it did not stain any lymphatic endothelial cells in normal tissues. PMab-38 could be useful for uncovering the function of PDPN in canine melanomas.

KEYWORDS:

canine podoplanin; immunohistochemistry; melanoma; monoclonal antibody

PMID:
27918691
PMCID:
PMC5206701
DOI:
10.1089/mab.2016.0040
[Indexed for MEDLINE]
Free PMC Article

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