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Sci Rep. 2016 Dec 5;6:36761. doi: 10.1038/srep36761.

Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm.

Author information

1
Department of Food Science and Nutrition, College of Food and Agriculture, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
2
College of Science &General Studies, Alfaisal University, Riyadh 11533, Kingdom of Saudi Arabia.
3
Department of Chemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
4
Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh-202002, India.
5
Protein Research Chair, Department of Biochemistry, College of Science, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
6
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh-11451, Kingdom of Saudi Arabia.
7
Department of Botany, Aligarh Muslim University, Aligarh-202002, India.
8
Department of Zoology, College of Sciences, King Saud University, Riyadh, Saudi Arabia.
9
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
10
Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya St., 119991 Moscow, Russia.
11
GALLY International Biomedical Research Consulting LLC., 7733 Louis Pasteur Drive, #330, San Antonio, TX, 78229, USA.
12
School of Health Science and Healthcare Administration, University of Atlanta, E. Johns Crossing, #175, Johns Creek, GA, 30097, USA.
13
Institute of Physiologically Active Compounds Russian Academy of Sciences, Chernogolovka, 142432, Russia.

Abstract

Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs). Synthesized nanostructures were characterized hexagonal wurtzite structure of size ~24 nm by UV-visible, XRD, FTIR and TEM. NS-ZnNPs demonstrated broad-spectrum QS inhibition in C. violaceum and P. aeruginosa biosensor strains. Synthesized nanostructures inhibited QS regulated functions of C. violaceum CVO26 (violacein) and elastase, protease, pyocyanin and alginate production in PAO1 significantly. NS-ZnNPs at sub-inhibitory concentrations inhibited the biofilm formation of four-food pathogens viz. C. violaceum 12472, PAO1, L. monocytogenes, E. coli. Moreover, NS-ZnNPs was found effective in inhibiting pre-formed mature biofilms of the four pathogens. Therefore, the broad-spectrum inhibition of QS and biofilm by biogenic Zinc oxide nanoparticles and it is envisaged that these nontoxic bioactive nanostructures can be used as food packaging material and/or as food preservative.

PMID:
27917856
PMCID:
PMC5137238
DOI:
10.1038/srep36761
[Indexed for MEDLINE]
Free PMC Article

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