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Front Microbiol. 2016 Nov 18;7:1858. eCollection 2016.

The Absence of the N-acyl-homoserine-lactone Autoinducer Synthase Genes traI and ngrI Increases the Copy Number of the Symbiotic Plasmid in Sinorhizobium fredii NGR234.

Author information

  • 1Department of Microbiology and Biotechnology, Biocenter Klein Flottbek, University of Hamburg Hamburg, Germany.
  • 2Core Unit Systems Medicine, University of Würzburg Würzburg, Germany.
  • 3Plant Genetics Research Unit, Agricultural Research Service, United States Department of Agriculture, University of Missouri Columbia, MO, USA.
  • 4Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance Johann Wolfgang Goethe-University Frankfurt, Germany.

Abstract

Plant-released flavonoids induce the transcription of symbiotic genes in rhizobia and one of the first bacterial responses is the synthesis of so called Nod factors. They are responsible for the initial root hair curling during onset of root nodule development. This signal exchange is believed to be essential for initiating the plant symbiosis with rhizobia affiliated with the Alphaproteobacteria. Here, we provide evidence that in the broad host range strain Sinorhizobium fredii NGR234 the complete lack of quorum sensing molecules results in an elevated copy number of its symbiotic plasmid (pNGR234a). This in turn triggers the expression of symbiotic genes and the production of Nod factors in the absence of plant signals. Therefore, increasing the copy number of specific plasmids could be a widespread mechanism of specialized bacterial populations to bridge gaps in signaling cascades.

KEYWORDS:

RNA sequencing (RNA-Seq); Sinorhizobium fredii; plant symbioses; plasmid copy number; quorum sensing (QS)

PMID:
27917168
PMCID:
PMC5114275
DOI:
10.3389/fmicb.2016.01858
[PubMed - in process]
Free PMC Article
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