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Int J Mol Sci. 2016 Nov 30;17(12). pii: E1999.

Coriandrum sativum and Lavandula angustifolia Essential Oils: Chemical Composition and Activity on Central Nervous System.

Author information

1
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. lcaputo@unisa.it.
2
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. luceia.souza@ufrgs.br.
3
Department of Agronomy, University of Rio Grande do Sul (UFRGS), 91501-970 Porto Alegre, Brazil. luceia.souza@ufrgs.br.
4
ETT Spa, via Sestri 37, 16154 Genova, Italy. susanna.alloisio@ettsolutions.com.
5
Dipartimento di Scienze della Terra, dell'Ambiente e della Vita, University of Genoa, Corso Europa 26, 16132 Genoa, Italy. cornara@dipteris.unige.it.
6
Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano (Salerno), Italy. defeo@unisa.it.

Abstract

The aims of this study are to determine the chemical composition of Lavandula angustifolia Mill. and Coriandrum sativum L. essential oils, to evaluate their cytotoxic effects in SH-SY5Y human neuroblastoma cells, to investigate whether an alteration of adenylate cyclase 1 (ADCY1) and of extracellular signal-regulated kinase (ERK) expression can take part in the molecular mechanisms of the essential oils, and to study their possible neuronal electrophysiological effects. The essential oils were obtained by hydrodistillation, and studied by GC and GC-MS. In the oils from L. angustifolia and C. sativum, linalool was the main component (33.1% and 67.8%, respectively). SH-SY5Y cells were incubated with different concentrations of essential oils and of linalool. Cell viability and effects on ADCY1 and ERK expression were analyzed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT and Western blotting, respectively. Variation in cellular electrophysiology was studied in primary cultures of rat cortical neurons with a multi-electrode array (MEA)-based approach. The essential oils and linalool revealed different cytotoxic activities. Linalool inhibited ADCY1 and ERK expression. Neuronal networks subjected to L. angustifolia and C. sativum essential oils showed a concentration-dependent inhibition of spontaneous electrical activity.

KEYWORDS:

Adenylyl Cyclase (ADCY); Central Nervous System (CNS); Coriandrum sativum; Extracellular Signal-Regulated Kinase (ERK); Lavandula angustifolia; Multi Electrode Array (MEA); cytotoxicity; essential oil; linalool

PMID:
27916876
PMCID:
PMC5187799
DOI:
10.3390/ijms17121999
[Indexed for MEDLINE]
Free PMC Article

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