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Oncotarget. 2017 Jan 10;8(2):2400-2412. doi: 10.18632/oncotarget.13681.

Glutamine and glutaminolysis are required for efficient replication of infectious spleen and kidney necrosis virus in Chinese perch brain cells.

Fu X1,2,3, Hu X2,4, Li N1,2, Zheng F2, Dong X2, Duan J2, Lin Q1,2, Tu J2, Zhao L2, Huang Z1, Su J2,3, Lin L2,5.

Author information

1
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal Immune Technology, Guangdong Provinces, Guangzhou, Guangdong, 510380, China.
2
Department of Aquatic Animal Medicine, Research Center of Marine Biology, College of Fisheries, Freshwater Aquaculture Collaborative Innovation Center of Hubei Province Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
3
College of Animal Science and Technology, Northwest A and F University, Shanxi Key Laboratory of Molecular Biology for Aquaculture, Yangling, 712100, China.
4
School of Animal Sciences and Nutritional Engineering, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China.
5
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan Provincial Key Laboratory for Tropical Hydrobiology and Biotechnology, College of Marine Science, Hainan University, Haikou 570228, China.

Abstract

Viruses rely on host cellular metabolism for energy and macromolecule synthesis during their replication. Infectious spleen and kidney necrosis virus (ISKNV) causes significant economic losses in the Chinese perch (Siniperca chuatsi) industry worldwide. However, little is known about the relationship between ISKNV replication and cellular metabolism. Using transcriptomic analysis, we observed that glutamine metabolism in Chinese perch brain (CPB) cells is altered during ISKNV infection. Moreover, ISKNV replication was decreased in CPB cells cultured in the glutamine-depleted medium. ISKNV replication was also inhibited in CPB cells cultured in the presence of bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (an inhibitor of glutaminase), (-)-epigallocatechinmo nogallate (an inhibitor of glutamate dehydrogenase) or L-buthionine sulfoximine (an inhibitor of glutathione synthesis). However, virus replication was rescued by the addition of multiple tricarboxylic acid cycle intermediates, ATP, or glutathione reduced ethyl ester. ATP and reduced glutathione/oxidized glutathione levels were increased in CPB cells infected with ISKNV, but were decreased in CPB cells cultured in glutamine-depleted medium. These results indicate ISKNV infection induces glutaminolysis to accommodate the biosynthetic and energy needs for its efficient virus replication.

KEYWORDS:

ISKNV; Siniperca chuatsi; TCA cycle; glutamine; glutaminolysis

PMID:
27911855
PMCID:
PMC5356810
DOI:
10.18632/oncotarget.13681
[Indexed for MEDLINE]
Free PMC Article

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