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Angew Chem Int Ed Engl. 2017 Jan 2;56(1):193-197. doi: 10.1002/anie.201609269. Epub 2016 Dec 2.

Cloaked Caged Compounds: Chemical Probes for Two-Photon Optoneurobiology.

Author information

1
Department of Neuroscience, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY, USA.

Abstract

Caged neurotransmitters, in combination with focused light beams, enable precise interrogation of neuronal function, even at the level of single synapses. However, most caged transmitters are, surprisingly, severe antagonists of ionotropic gamma-aminobutyric acid (GABA) receptors. By conjugation of a large, neutral dendrimer to a caged GABA probe we introduce a "cloaking" technology that effectively reduces such antagonism to very low levels. Such cloaked caged compounds will enable the study of the signaling of the inhibitory neurotransmitter GABA in its natural state using two-photon uncaging microscopy for the first time.

KEYWORDS:

GABA-A receptors; biologically inert; caged compounds; optical methods; two-photon photolysis

PMID:
27910251
PMCID:
PMC5195861
DOI:
10.1002/anie.201609269
[Indexed for MEDLINE]
Free PMC Article

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