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Methods Mol Biol. 2017;1541:43-58.

Cytogenetic Harvesting of Cancer Cells and Cell Lines.

Author information

  • 1Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures, Leibniz Institute-DSMZ, Inhoffenstr. 7b, 38124, Braunschweig, Germany. rml@dsmz.de.
  • 2Department of Human and Animal Cell Lines, German Collection of Microorganisms and Cell Cultures, Leibniz Institute-DSMZ, Inhoffenstr. 7b, 38124, Braunschweig, Germany.

Abstract

We describe an evidence-based approach toward optimizing chromosome preparation from cancer cells and cell lines. The procedures described here emphasize the utility of both cell culture-to maximize the yields of the dividing cells needed to harvest mitotic metaphase chromosome preparations and how an empirical evaluation of hypotonic treatments enables optimal conditions to be efficiently determined.

KEYWORDS:

Cancer chromosomes; Cell culture; Fixation; Hypotonic treatment; Slide making

PMID:
27910013
DOI:
10.1007/978-1-4939-6703-2_5
[PubMed - in process]
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