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World J Microbiol Biotechnol. 2017 Jan;33(1):19. Epub 2016 Nov 30.

Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications.

Kang Z1,2, Huang H3,4, Zhang Y3,4, Du G3,4, Chen J3,4.

Author information

1
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. zkang@jiangnan.edu.cn.
2
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, Jiangsu, China. zkang@jiangnan.edu.cn.
3
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
4
Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, 214122, Jiangsu, China.

Abstract

Pichia pastoris: (reclassified as Komagataella phaffii), a methylotrophic yeast strain has been widely used for heterologous protein production because of its unique advantages, such as readily achievable high-density fermentation, tractable genetic modifications and typical eukaryotic post-translational modifications. More recently, P. pastoris as a metabolic pathway engineering platform has also gained much attention. In this mini-review, we addressed recent advances of molecular toolboxes, including synthetic promoters, signal peptides, and genome engineering tools that established for P. pastoris. Furthermore, the applications of P. pastoris towards synthetic biology were also discussed and prospected especially in the context of genome-scale metabolic pathway analysis.

KEYWORDS:

Biopharmaceuticals; Cell factory; Metabolic engineering; Molecular toolbox; Pichia pastoris; Synthetic biology

PMID:
27905091
DOI:
10.1007/s11274-016-2185-2
[Indexed for MEDLINE]

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