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Methods Mol Biol. 2017;1539:1-12.

Construction of Small-Insert and Large-Insert Metagenomic Libraries.

Author information

1
Pohl-Boskamp GmbH, Hamburg, Germany.
2
Göttingen Genomics Laboratory, Department of Genomic and Applied Microbiology, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Grisebachstr. 8, 37077, Göttingen, Germany. rdaniel@gwdg.de.

Abstract

The vast majority of the Earth's biological diversity is hidden in uncultured and yet uncharacterized microbial genomes. The construction of metagenomic libraries is a cultivation-independent molecular approach to assess this unexplored genetic reservoir. High numbers of novel biocatalysts have been identified by function-based or sequence-based screening of metagenomic libraries derived from various environments. Here, we describe detailed protocols for the construction of metagenomic small-insert and large-insert libraries in plasmids and fosmids, respectively, from environmental DNA.

KEYWORDS:

Fosmid; Large-insert library; Metagenomic DNA; Plasmid; Small-insert library; WGA; Whole genome amplification

PMID:
27900680
DOI:
10.1007/978-1-4939-6691-2_1
[Indexed for MEDLINE]

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