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Oncol Lett. 2016 Nov;12(5):3543-3548. Epub 2016 Sep 5.

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer.

Author information

1
Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China; Department of Graduate Studies, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.
2
Central Laboratory, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China.
3
Department of Biochemistry and Molecular Biology, Zhuhai Campus Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.

Abstract

Previous studies have indicated that the deregulation of microRNAs contributes to tumorigenesis. Misregulation of microRNA-520e (miR-520e) has been observed in various types of cancer. However, the expression profile and biological function of miR-520e in breast cancer remains largely unknown. The present study demonstrated that miR-520e expression was significantly increased in breast cancer tissues compared with adjacent non-cancerous breast tissues in 21 patients, as revealed by reverse transcription-quantitative polymerase chain reaction. Furthermore, the proliferation capacity of breast cancer cells was markedly enhanced by the introduction of miR-520e in vitro using a cell counting kit-8 assay. The present study also revealed that the overexpression of miR-520e could suppress breast cancer cell apoptosis, revealed using Annexin V/propidium iodide double staining and flow cytometry analysis. In addition, the ectopic expression of miR-520e promoted the migration of breast cancer cells in vitro, as demonstrated by a Transwell assay. Overall, the findings of the present study highlight an important role for miR-520e in breast cancer development and in the molecular etiology of breast cancer, which indicates the potential application of miR-520e in cancer therapy.

KEYWORDS:

apoptosis; breast cancer; miRNA-520e; migration; proliferation

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