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Cancer Res. 2017 Feb 1;77(3):766-779. doi: 10.1158/0008-5472.CAN-16-1346. Epub 2016 Nov 29.

Transcriptional Regulator CNOT3 Defines an Aggressive Colorectal Cancer Subtype.

Author information

1
Department of Medical Oncology and Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, Massachusetts.
2
Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
3
Department of Medical Oncology, Hospital La Paz Institute for Health Research CIBERONC, Madrid, Spain.
4
Pathology Institute, Ludwig-Maximilians-Universitat, Munich, Germany.
5
Department of Pathology, Hospital La Paz Institute for Health Research, Madrid, Spain.
6
Center for Gastrointestinal Cancer Research, Baylor University Medical Center, Dallas, Texas.
7
Department of Medical Oncology and Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, Massachusetts. ramesh_shivdasani@dfci.harvard.edu.

Abstract

Cancer cells exhibit dramatic alterations of chromatin organization at cis-regulatory elements, but the molecular basis, extent, and impact of these alterations are still being unraveled. Here, we identify extensive genome-wide modification of sites bearing the active histone mark H3K4me2 in primary human colorectal cancers, as compared with corresponding benign precursor adenomas. Modification of certain colorectal cancer sites highlighted the activity of the transcription factor CNOT3, which is known to control self-renewal of embryonic stem cells (ESC). In primary colorectal cancer cells, we observed a scattered pattern of CNOT3 expression, as might be expected for a tumor-initiating cell marker. Colorectal cancer cells exhibited nuclear and cytoplasmic expression of CNOT3, suggesting possible roles in both transcription and mRNA stability. We found that CNOT3 was bound primarily to genes whose expression was affected by CNOT3 loss, and also at sites modulated in certain types of colorectal cancers. These target genes were implicated in ESC and cancer self-renewal and fell into two distinct groups: those dependent on CNOT3 and MYC for optimal transcription and those repressed by CNOT3 binding and promoter hypermethylation. Silencing CNOT3 in colorectal cancer cells resulted in replication arrest. In clinical specimens, early-stage tumors that included >5% CNOT3+ cells exhibited a correlation to worse clinical outcomes compared with tumors with little to no CNOT3 expression. Together, our findings implicate CNOT3 in the coordination of colonic epithelial cell self-renewal, suggesting this factor as a new biomarker for molecular and prognostic classification of early-stage colorectal cancer. Cancer Res; 77(3); 766-79.

PMID:
27899379
PMCID:
PMC5290184
DOI:
10.1158/0008-5472.CAN-16-1346
[Indexed for MEDLINE]
Free PMC Article

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