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Dev Cell. 2016 Dec 5;39(5):597-610. doi: 10.1016/j.devcel.2016.10.020. Epub 2016 Nov 23.

The Red Light Receptor Phytochrome B Directly Enhances Substrate-E3 Ligase Interactions to Attenuate Ethylene Responses.

Author information

1
State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, Beijing 100871, China.
2
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
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Contributed equally

Abstract

Plants germinating under subterranean darkness assume skotomorphogenesis, a developmental program strengthened by ethylene in response to mechanical pressure of soil. Upon reaching the surface, light triggers a dramatic developmental transition termed de-etiolation that requires immediate termination of ethylene responses. Here, we report that light activation of photoreceptor phyB results in rapid degradation of EIN3, the master transcription factor in the ethylene signaling pathway. As a result, light rapidly and efficiently represses ethylene actions. Specifically, phyB directly interacts with EIN3 in a light-dependent manner and also physically associates with F box protein EBFs. The light-activated association of phyB, EIN3, and EBF1/EBF2 proteins stimulates robust EIN3 degradation by SCFEBF1/EBF2 E3 ligases. We reveal that phyB manipulates substrate-E3 ligase interactions in a light-dependent manner, thus directly controlling the stability of EIN3. Our findings illustrate a mechanistic model of how plants transduce light information to immediately turn off ethylene signaling for de-etiolation initiation.

KEYWORDS:

EBF1 and EBF2; EIN3; ethylene signaling; light signaling; photomorphogenesis; phytochrome B; seedling emergence; skotomorphogenesis

PMID:
27889482
PMCID:
PMC5140706
DOI:
10.1016/j.devcel.2016.10.020
[Indexed for MEDLINE]
Free PMC Article

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