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Biochim Biophys Acta Mol Cell Res. 2017 Feb;1864(2):367-381. doi: 10.1016/j.bbamcr.2016.11.020. Epub 2016 Nov 23.

Rab7a regulates cell migration through Rac1 and vimentin.

Author information

1
Department of Biological and Environmental Sciences and Technologies, (DiSTeBA) University of Salento, Via Provinciale Monteroni 165, 73100 Lecce, Italy; Department of Biosciences, Centre for Immune Regulation, University of Oslo, Blindernveien 31, 0371 Oslo, Norway.
2
Department of Biosciences, Centre for Immune Regulation, University of Oslo, Blindernveien 31, 0371 Oslo, Norway. Electronic address: c.a.m.progida@ibv.uio.no.
3
Department of Biosciences, Centre for Immune Regulation, University of Oslo, Blindernveien 31, 0371 Oslo, Norway. Electronic address: oddmund.bakke@ibv.uio.no.
4
Department of Biological and Environmental Sciences and Technologies, (DiSTeBA) University of Salento, Via Provinciale Monteroni 165, 73100 Lecce, Italy. Electronic address: cecilia.bucci@unisalento.it.

Abstract

Rab7a, a small GTPase of the Rab family, is localized to late endosomes and controls late endocytic trafficking. The discovery of several Rab7a interacting proteins revealed that Rab7a function is closely connected to cytoskeletal elements. Indeed, Rab7a recruits on vesicles RILP and FYCO that are responsible for the movement of Rab7a-positive vesicles and/or organelles on microtubule tracks, but also directly interacts with Rac1, a fundamental regulator of actin cytoskeleton, and with peripherin and vimentin, two intermediate filament proteins. Considering all these interactions and, in particular, the fact that Rac1 and vimentin are key factors for cellular motility, we investigated a possible role of Rab7a in cell migration. We show here that Rab7a is needed for cell migration as Rab7a depletion causes slower migration of NCI H1299 cells affecting cell velocity and directness. Rab7a depletion negatively affects adhesion and spreading onto fibronectin substrates, altering β1-integrin activation, localization and intracellular trafficking, and myosin X localization. In fact, Rab7a-depleted cells show 40% less filopodia and active integrin accumulates at the leading edge of migrating cells. Furthermore, Rab7a depletion decreases the amount of active Rac1 but not its abundance and reduces the number of cells with vimentin filaments facing the wound, indicating that Rab7a has a role in the orientation of vimentin filaments during migration. In conclusion, our results demonstrate a key role of Rab7a in the regulation of different aspects of cell migration.

KEYWORDS:

Cell migration; Myosin X; Rab7a; Rac1; Vimentin; β1-integrin

PMID:
27888097
DOI:
10.1016/j.bbamcr.2016.11.020
[Indexed for MEDLINE]
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