Probes for manipulating and monitoring IP3

Akitoshi Miyamoto; Katsuhiko Mikoshiba

doi:10.1016/j.ceca.2016.11.003

Probes for manipulating and monitoring IP₃

Cell Calcium. 2017 Jun:64:57-64. doi: 10.1016/j.ceca.2016.11.003. Epub 2016 Nov 17.

Authors

Akitoshi Miyamoto¹, Katsuhiko Mikoshiba²

Affiliations

¹ Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
² Laboratory for Developmental Neurobiology, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. Electronic address: mikosiba@brain.riken.jp.

PMID: 27887748
DOI: 10.1016/j.ceca.2016.11.003

Abstract

Inositol 1,4,5-trisphosphate (IP₃) is an important second messenger produced via G-protein-coupled receptor- or receptor tyrosine kinase-mediated pathways. IP₃ levels induce Ca²⁺ release from the endoplasmic reticulum (ER) via IP₃ receptor (IP₃R) located in the ER membrane. The resultant spatiotemporal pattern of Ca²⁺ signals regulates diverse cellular functions, including fertilization, gene expression, synaptic plasticity, and cell death. Therefore, monitoring and manipulating IP₃ levels is important to elucidate not only the functions of IP₃-mediated pathways but also the encoding mechanism of IP₃R as a converter of intracellular signals from IP₃ to Ca²⁺.

Keywords: IP(3) induced Ca(2+) response; IP(3) probes.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism
Fluorescent Dyes / metabolism
Humans
Inositol 1,4,5-Trisphosphate / metabolism*
Molecular Probes / metabolism*
Optogenetics
Signal Transduction

Substances

Fluorescent Dyes
Molecular Probes
Inositol 1,4,5-Trisphosphate
Calcium