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Nutrients. 2016 Nov 24;8(12). pii: E728.

Sodium Butyrate Reduces Colitogenic Immunoglobulin A-Coated Bacteria and Modifies the Composition of Microbiota in IL-10 Deficient Mice.

Zhang T1,2, Ding C3, Zhao M4, Dai X5, Yang J6, Li Y7, Gu L8, Wei Y9, Gong J10,11, Zhu W12, Li N13, Li J14.

Author information

1
Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. tenghuiff@163.com.
2
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. tenghuiff@163.com.
3
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dingchao19910521@126.com.
4
Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. zhao_ming_li@163.com.
5
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. d670677094@163.com.
6
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. yang_jianbo@126.com.
7
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. liyi1860@126.com.
8
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. drgulili@163.com.
9
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dr_betty@126.com.
10
Department of General Surgery, Jinling Hospital Affiliated to Southern Medical University, No. 305 East Zhongshan Rd., Nanjing 210002, China. gongjianfeng@aliyun.com.
11
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. gongjianfeng@aliyun.com.
12
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. dr_zhuweiming@126.com.
13
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. proflining@163.com.
14
Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, China. lijieshou@163.com.

Abstract

High levels of immunoglobulin A (IgA)-coated bacteria may have a role in driving inflammatory bowel disease (IBD). We therefore investigated the effect of sodium butyrate on microbiota in IBD prone interleukin (IL)-10-/- mice. At 8 weeks of age, mice were allocated into three groups (n = 4/group): normal (C57BL/6), IL-10-/-, and IL-10-/- treated with sodium butyrate (100 mM). Severity of colitis, inflammatory cytokine and short-chain fatty acid (SCFA) concentration in proximal colon contents, the percentage of IgA-coated bacteria and microbiota composition by 16S ribosomal RNA assessment of stool were measured after 4 weeks of treatment. Sodium butyrate ameliorated histological colitis and decreased levels of tumor necrosis factor (TNF)-α and IL-6 in IL-10-/- mice compared with those without treatment. At the phylum level, a reduction in Bacteroidetes and an increase in Firmicutes in IL-10-/- mice treated with sodium butyrate were observed. Additionally, Prevotellaceae species were reduced in IL-10-/- mice treated with sodium butyrate as compared with those without treatment. The level of biodiversity was slightly increased and the amount of IgA-coated bacteria decreased in IL-10-/- mice treated with sodium butyrate compared with those without treatment. Our results indicate that sodium butyrate protects against colitis, possibly through modifying the gut microbiota, enriching biodiversity and reducing the amount of colitogenic IgA-coated bacteria in IL-10-/- mice.

KEYWORDS:

IL-10−/− mice; colitis; immunoglobulin A-coated bacteria; microbiota composition; sodium butyrate

PMID:
27886121
PMCID:
PMC5188405
DOI:
10.3390/nu8120728
[Indexed for MEDLINE]
Free PMC Article

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