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Plant Physiol. 2017 Jan;173(1):582-599. doi: 10.1104/pp.16.01602. Epub 2016 Nov 22.

Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.

Boruc J1,2,3,4,5,6,7, Weimer AK8,9,10,11,12,13,14, Stoppin-Mellet V8,9,10,11,12,13,14, Mylle E8,9,10,11,12,13,14, Kosetsu K8,9,10,11,12,13,14, Cedeño C8,9,10,11,12,13,14, Jaquinod M8,9,10,11,12,13,14, Njo M8,9,10,11,12,13,14, De Milde L8,9,10,11,12,13,14, Tompa P8,9,10,11,12,13,14, Gonzalez N8,9,10,11,12,13,14, Inzé D8,9,10,11,12,13,14, Beeckman T8,9,10,11,12,13,14, Vantard M8,9,10,11,12,13,14, Van Damme D1,2,3,4,5,6,7.

Author information

1
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium (J.B., A.K.W., E.M., K.K., M.N., L.D.M., N.G., D.I., T.B., D.V.D.); daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
2
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (J.B., A.K.W., E.M., K.K., M.N., L.D.M., N.G., D.V.D., D.I., T.B.); daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
3
Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, UMR5168, Centre National de la Recherche Scientifique/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Institut National de la Recherche Agronomique/Université Joseph-Fourier, Grenoble, France (V.S.-M.; M.V.); daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
4
Institut National de la Santé et de la Recherche Médicale, U836, F-38000 Grenoble, France (V.S.-M., M.V.); daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
5
Structural Biology Research Center, VIB, 1050 Brussels, Belgium (C.C., P.T.); daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
6
Structural Biology Brussels, Vrije Universiteit Brussels, 1050 Brussels, Belgium (C.C., P.T.); and daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
7
Exploring the Dynamics of Proteomes Laboratoire Biologie à Grande Echelle, U1038 Institut National de la Santé et de la Recherche Médicale/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Université Joseph-Fourier Institut de Recherches en Technologies et Sciences pour le Vivant/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Grenoble, F-38054 Grenoble Cedex 9, France (M.J.) daniel.vandamme@psb.vib-ugent.be joanna.boruc@psb.vib-ugent.be.
8
Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium (J.B., A.K.W., E.M., K.K., M.N., L.D.M., N.G., D.I., T.B., D.V.D.).
9
Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (J.B., A.K.W., E.M., K.K., M.N., L.D.M., N.G., D.V.D., D.I., T.B.).
10
Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherches en Technologies et Sciences pour le Vivant, UMR5168, Centre National de la Recherche Scientifique/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Institut National de la Recherche Agronomique/Université Joseph-Fourier, Grenoble, France (V.S.-M.; M.V.).
11
Institut National de la Santé et de la Recherche Médicale, U836, F-38000 Grenoble, France (V.S.-M., M.V.).
12
Structural Biology Research Center, VIB, 1050 Brussels, Belgium (C.C., P.T.).
13
Structural Biology Brussels, Vrije Universiteit Brussels, 1050 Brussels, Belgium (C.C., P.T.); and.
14
Exploring the Dynamics of Proteomes Laboratoire Biologie à Grande Echelle, U1038 Institut National de la Santé et de la Recherche Médicale/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Université Joseph-Fourier Institut de Recherches en Technologies et Sciences pour le Vivant/Commissariat à l'Énergie Atomique et aux Énergies Alternatives/Grenoble, F-38054 Grenoble Cedex 9, France (M.J.).

Abstract

Aurora kinases are key effectors of mitosis. Plant Auroras are functionally divided into two clades. The alpha Auroras (Aurora1 and Aurora2) associate with the spindle and the cell plate and are implicated in controlling formative divisions throughout plant development. The beta Aurora (Aurora3) localizes to centromeres and likely functions in chromosome separation. In contrast to the wealth of data available on the role of Aurora in other kingdoms, knowledge on their function in plants is merely emerging. This is exemplified by the fact that only histone H3 and the plant homolog of TPX2 have been identified as Aurora substrates in plants. Here we provide biochemical, genetic, and cell biological evidence that the microtubule-bundling protein MAP65-1-a member of the MAP65/Ase1/PRC1 protein family, implicated in central spindle formation and cytokinesis in animals, yeasts, and plants-is a genuine substrate of alpha Aurora kinases. MAP65-1 interacts with Aurora1 in vivo and is phosphorylated on two residues at its unfolded tail domain. Its overexpression and down-regulation antagonistically affect the alpha Aurora double mutant phenotypes. Phospho-mutant analysis shows that Aurora contributes to the microtubule bundling capacity of MAP65-1 in concert with other mitotic kinases.

PMID:
27879390
PMCID:
PMC5210758
DOI:
10.1104/pp.16.01602
[Indexed for MEDLINE]
Free PMC Article

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