Stem Cell Res. 2016 Sep;17(2):444-447. doi: 10.1016/j.scr.2016.09.015. Epub 2016 Sep 17.

An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts.

Sosa E¹, Kim R¹, Rojas EJ¹, Hosohama L¹, Hennebold JD², Orwig KE³, Clark AT⁴.

Author information

1: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
2: Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97006, USA.
3: Department of Obstetrics, Gynecology and Reproductive Sciences and Magee-Womans Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
4: Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: clarka@ucla.edu.

Abstract

We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.