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Biotechnol Lett. 2017 Mar;39(3):359-366. doi: 10.1007/s10529-016-2260-7. Epub 2016 Nov 23.

Single-cell RNA-seq reveals lincRNA expression differences in Hela-S3 cells.

Wang J1,2,3, Roy B4,5,6.

Author information

1
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China. wangjie1@genomics.cn.
2
BGI-Shenzhen, Shenzhen, 518083, China. wangjie1@genomics.cn.
3
BGI Genomics Co. Ltd., Shenzhen, 518083, China. wangjie1@genomics.cn.
4
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, 518083, China.
5
BGI-Shenzhen, Shenzhen, 518083, China.
6
BGI Genomics Co. Ltd., Shenzhen, 518083, China.

Abstract

OBJECTIVE:

To characterize transcriptome-wide lincRNAs of Hela-S3 cell line by analyzing RNA sequencing data to provide a foundation for further functional verification and clinical application of cervical carcinoma development.

RESULTS:

Single-cell RNA sequencing data of 37 Hela-S3 cells were analysed. On average, 511 lincRNAs were expressed in each cell. Comparing the expression difference of the lincRNAs and protein-coding genes, we found that lincRNAs expression displayed more cell specificity than that of protein-coding genes (t-test, P<2.2E-16). In co-expression network analysis, we identified seven modules and one of them was enriched in pathways of mitotic, packaging of telomere ends, and chromosome maintenance.

CONCLUSION:

incRNAs are specifically expressed and form a network to perform function at single cell level. Their expression was more specific than that of protein-coding genes.

KEYWORDS:

Cervical carcinoma; Hela cell line; LincRNAs; RNA-seq

PMID:
27878653
DOI:
10.1007/s10529-016-2260-7
[Indexed for MEDLINE]

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