Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

Shogo Tsuji; Shiroh Futaki; Miki Imanishi

doi:10.1039/c6cc06824c

Sequence-specific recognition of methylated DNA by an engineered transcription activator-like effector protein

Chem Commun (Camb). 2016 Dec 6;52(99):14238-14241. doi: 10.1039/c6cc06824c.

Authors

Shogo Tsuji¹, Shiroh Futaki¹, Miki Imanishi¹

Affiliation

¹ Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. imiki@scl.kyoto-u.ac.jp.

PMID: 27872906
DOI: 10.1039/c6cc06824c

Abstract

A 5mC-selective TALE-repeat was created by screening a TALE repeat library containing randomized amino acids at repeat variable diresidues and their neighboring residues. The new repeat showed high 5mC discrimination ability. An artificial TALE containing the new repeat activated an endogenous gene in a genomic methylation status-dependent manner.

MeSH terms

Amino Acid Sequence
Amino Acids / chemistry
Amino Acids / genetics*
DNA Methylation / genetics*
Genetic Engineering*
Transcription Activator-Like Effectors / chemistry
Transcription Activator-Like Effectors / genetics*
Transcription Activator-Like Effectors / metabolism

Substances

Amino Acids
Transcription Activator-Like Effectors