Format

Send to

Choose Destination
J Histochem Cytochem. 2017 Jan;65(1):47-58. doi: 10.1369/0022155416678182. Epub 2016 Nov 23.

Unique Cellular Lineage Composition of the First Gland of the Mouse Gastric Corpus.

Author information

1
Department of Surgery (AO, EC, ACE, JRG), Vanderbilt University Medical Center, Nashville, Tennessee.
2
Epithelial Biology Center (CPP, EC, ACE, JRG), Vanderbilt University Medical Center, Nashville, Tennessee.
3
Nashville VA Medical Center (EC, JRG), Vanderbilt University Medical Center, Nashville, Tennessee.
4
Department of Cell and Developmental Biology (CPP, JRG), Vanderbilt University Medical Center, Nashville, Tennessee.

Abstract

The glandular stomach has two major zones: the acid secreting corpus and the gastrin cell-containing antrum. Nevertheless, a single gland lies at the transition between the forestomach and corpus in the mouse stomach. We have sought to define the lineages that make up this gland unit at the squamocolumnar junction. The first gland in mice showed a notable absence of characteristic corpus lineages, including parietal cells and chief cells. In contrast, the gland showed strong staining of Griffonia simplicifolia-II (GSII)-lectin-positive mucous cells at the bases of glands, which were also positive for CD44 variant 9 and Clusterin. Prominent numbers of doublecortin-like kinase 1 (DCLK1) positive tuft cells were present in the first gland. The first gland contained Lgr5-expressing putative progenitor cells, and a large proportion of the cells were positive for Sox2. The cells of the first gland stained strongly for MUC4 and EpCAM, but both were absent in the normal corpus mucosa. The present studies indicate that the first gland in the corpus represents a unique anatomic entity. The presence of a concentration of progenitor cells and sensory tuft cells in this gland suggests that it may represent a source of reserve reparative cells for adapting to severe mucosal damage.

KEYWORDS:

Clusterin; Lgr5; MUC4; Sox2; brush cell; tuft cell

PMID:
27872404
PMCID:
PMC5256201
DOI:
10.1369/0022155416678182
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center